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Literature summary extracted from

  • Wang, P.; Wu, P.; Myers, J.G.; Stamford, A.; Egan, R.W.; Billah, M.M.
    Characterization of human, dog and rabbit corpus cavernosum type 5 phosphodiesterases (2001), Life Sci., 68, 1977-1987.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.35 3-isobutyl-1-methylxanthine 0.0058 mM, 50% inhibition Canis lupus familiaris
3.1.4.35 3-isobutyl-1-methylxanthine 0.0058 mM, 50% inhibition Homo sapiens
3.1.4.35 3-isobutyl-1-methylxanthine 0.0054 mM, 50% inhibition Oryctolagus cuniculus
3.1.4.35 dipyridamole 0.0013 mM, 50% inhibition Canis lupus familiaris
3.1.4.35 dipyridamole 0.00075 mM, 50% inhibition Homo sapiens
3.1.4.35 dipyridamole 0.0012 mM, 50% inhibition Oryctolagus cuniculus
3.1.4.35 sildenafil 0.0000017 mM, 50% inhibition Canis lupus familiaris
3.1.4.35 sildenafil 0.0000036 mM, 50% inhibition Homo sapiens
3.1.4.35 sildenafil 0.000003 mM, 50% inhibition Oryctolagus cuniculus
3.1.4.35 zaprinast 0.0002 mM, 50% inhibition Canis lupus familiaris
3.1.4.35 zaprinast 0.0003 mM, 50% inhibition Homo sapiens
3.1.4.35 zaprinast 0.000053 mM, 50% inhibition Oryctolagus cuniculus
3.1.4.35 Zn2+ complete inhibition above 0.1 mM Canis lupus familiaris
3.1.4.35 Zn2+ strong inhibition above 0.01 mM, almost complete inhibition at 0.1 mM Homo sapiens
3.1.4.35 Zn2+ strong inhibition above 0.01 mM, complete inhibition at 0.1 mM Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.35 0.0008
-
 cGMP 30°C, pH 7.4 Homo sapiens
3.1.4.35 0.001
-
 cGMP 30°C, pH 7.4, platelet PDE5 Homo sapiens
3.1.4.35 0.002
-
 cGMP 30°, pH 7.4 Canis lupus familiaris
3.1.4.35 0.0023
-
 cGMP 30°C, pH 7.4 Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.35 Mg2+ required for activity, approx. 1.5fold activation at 1 mM Oryctolagus cuniculus
3.1.4.35 Mg2+ required for activity, approx. 2.5fold activation at 1 mM Homo sapiens
3.1.4.35 Mg2+ required for activity, approx. 2fold activation at 1 mM Canis lupus familiaris
3.1.4.35 Zn2+ approx. 2.5 fold activation at 0.01 mM, strong inhibition above Homo sapiens
3.1.4.35 Zn2+ slight activation at 0.01 mM, strong inhibition above Canis lupus familiaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.35 guanosine 3',5'-cyclic phosphate + H2O Homo sapiens
-
 guanosine 5'-phosphate
-
 ?
3.1.4.35 guanosine 3',5'-cyclic phosphate + H2O Oryctolagus cuniculus
-
 guanosine 5'-phosphate
-
 ?
3.1.4.35 guanosine 3',5'-cyclic phosphate + H2O Canis lupus familiaris
-
 guanosine 5'-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.35 Canis lupus familiaris
-
-
-
3.1.4.35 Homo sapiens
-
-
-
3.1.4.35 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.35 Mono Q, partially purified Homo sapiens
3.1.4.35 Mono Q, partially purified Oryctolagus cuniculus
3.1.4.35 Mono Q, partially purified Canis lupus familiaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.35 corpus cavernosum
-
 Homo sapiens
-
3.1.4.35 corpus cavernosum
-
 Canis lupus familiaris
-
3.1.4.35 platelet
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.35 guanosine 3',5'-cyclic phosphate + H2O
-
 Homo sapiens guanosine 5'-phosphate
-
 ?
3.1.4.35 guanosine 3',5'-cyclic phosphate + H2O
-
 Oryctolagus cuniculus guanosine 5'-phosphate
-
 ?
3.1.4.35 guanosine 3',5'-cyclic phosphate + H2O
-
 Canis lupus familiaris guanosine 5'-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.35 PDE5
-
 Homo sapiens
3.1.4.35 PDE5
-
 Oryctolagus cuniculus
3.1.4.35 PDE5
-
 Canis lupus familiaris
3.1.4.35 type 5 phosphodiesterase
-
 Homo sapiens
3.1.4.35 type 5 phosphodiesterase
-
 Oryctolagus cuniculus
3.1.4.35 type 5 phosphodiesterase
-
 Canis lupus familiaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.35 7.5
-
-
 Homo sapiens
3.1.4.35 7.5
-
-
 Oryctolagus cuniculus
3.1.4.35 7.5
-
-
 Canis lupus familiaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.35 6 8.5 almost no activity at pH 6.0 and 8.5, respectively Homo sapiens
3.1.4.35 6.5 8.5 almost no activity at pH 6.5 and pH 8.5, respectively Canis lupus familiaris
3.1.4.35 6.5 8.5 very low activity at pH 6.5 and 8.5, respectively Oryctolagus cuniculus